the purpose of this study is to verify the interaction between food and ampicillin which is one of the aminopenicillins known to be absorbed by a specified dipeptide transporter in the small intestine. The absorption of ampicillin was measured in
the
presence of the high carbohydrate food, high fat food, and high protein food, and compared with that in the presence of the control normal food. In situ single-pass perfusion method was chosen in these experiments using two jejunal segments in
the
rat.
Reduction in the absorption of ampicillin was not show, when both high carbohydrate food and high fat food were co-perfused with ampicillin. When the high protein food was co-perfused with ampicillin, the difference of Cout/Cin of ampicillin
ratio
was
0.084±0.082, showing a trend of reduced absorption without a significance. Further, glcylsarcosine (Gly-Sar) which is a stable dipeptide in the small intestine was used in order to see the direct competitive inhibition with ampicillin on the
dipeptide
transporter. The difference of Cout/Cin ratio was 0.078±0.20 in the presence of 10mM Gly-Sar, showing a significant inhibition of ampicillin absorption (p<0.02). It suggests that dietary di-and tripeptides, the digestive products of protein
food,
might
have influence on the absorption of amicillin, and that ampicillin could be given at the fasting state for better absorption.
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